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Effects of RSV on mitochondrial function–related indicators exposed to TCS. (A, B) Protein expression and quantitative analysis of SIRT1, <t>PGC–1α,</t> NRF1, and mRNA expression levels (C) mRNA expression levels of SIRT1, PGC–1α, NRF1, and TFAM (D) mRNA expression levels of MFN2, OPA1, MFN1, DRP1, FIS1, and MFF. Data were analyzed using one–way ANOVA. Inter–group differences are indicated by different letters ( P < 0.05). Groups with the same letter were considered not to have significant differences (mean ± SD, n = 6).
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Effects of RSV on mitochondrial function–related indicators exposed to TCS. (A, B) Protein expression and quantitative analysis of SIRT1, <t>PGC–1α,</t> NRF1, and mRNA expression levels (C) mRNA expression levels of SIRT1, PGC–1α, NRF1, and TFAM (D) mRNA expression levels of MFN2, OPA1, MFN1, DRP1, FIS1, and MFF. Data were analyzed using one–way ANOVA. Inter–group differences are indicated by different letters ( P < 0.05). Groups with the same letter were considered not to have significant differences (mean ± SD, n = 6).
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Effects of RSV on mitochondrial function–related indicators exposed to TCS. (A, B) Protein expression and quantitative analysis of SIRT1, <t>PGC–1α,</t> NRF1, and mRNA expression levels (C) mRNA expression levels of SIRT1, PGC–1α, NRF1, and TFAM (D) mRNA expression levels of MFN2, OPA1, MFN1, DRP1, FIS1, and MFF. Data were analyzed using one–way ANOVA. Inter–group differences are indicated by different letters ( P < 0.05). Groups with the same letter were considered not to have significant differences (mean ± SD, n = 6).
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Effects of RSV on mitochondrial function–related indicators exposed to TCS. (A, B) Protein expression and quantitative analysis of SIRT1, <t>PGC–1α,</t> NRF1, and mRNA expression levels (C) mRNA expression levels of SIRT1, PGC–1α, NRF1, and TFAM (D) mRNA expression levels of MFN2, OPA1, MFN1, DRP1, FIS1, and MFF. Data were analyzed using one–way ANOVA. Inter–group differences are indicated by different letters ( P < 0.05). Groups with the same letter were considered not to have significant differences (mean ± SD, n = 6).
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Effects of RSV on mitochondrial function–related indicators exposed to TCS. (A, B) Protein expression and quantitative analysis of SIRT1, <t>PGC–1α,</t> NRF1, and mRNA expression levels (C) mRNA expression levels of SIRT1, PGC–1α, NRF1, and TFAM (D) mRNA expression levels of MFN2, OPA1, MFN1, DRP1, FIS1, and MFF. Data were analyzed using one–way ANOVA. Inter–group differences are indicated by different letters ( P < 0.05). Groups with the same letter were considered not to have significant differences (mean ± SD, n = 6).
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pgcs  (Servicebio Inc)
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In vitro isolation and culture process of <t>PGCs</t> from QYPCs with tva r2/r2 and tvb r3/r3 genotypes. (A) The process of isolating and initiating primary culture of PGCs from 6-7-day-old tva r2/r2 and tvb r3/r3 genotype QYPC embryos. (a) Fertile eggs and QYPCs with tva r2/r2 and tvb r3/r3 genotypes; (b) Embryos of 6-7 days old tva r2/r2 and tvb r3/r3 genotypes QYPCs; (c) a pair of male gonads attached to the surface of the kidney; (d) Several gonadal tissues that have been stripped clean; (e) somatic cells and PGCs after 5 h of differential adhesion; (f) Transfer the post-pasted PGCs to the cell culture plate for primary culture; (B) The Fig. shows the general process of co-culturing PGCs <t>with</t> <t>STO</t> cells for line establishment. (a) The cell morphology of PGCs-STO cells in primary co-culture for 4 days; (b) The cell morphology of PGCs-STO cells cocultured for 8 days; (c) The cell morphology of PGCs-STO cells cocultured for 14 days; (d) The cell morphology of PGCs-STO cells cocultured for 21days and successfully constructed.
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pgc  (Conviron Inc)
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In vitro isolation and culture process of <t>PGCs</t> from QYPCs with tva r2/r2 and tvb r3/r3 genotypes. (A) The process of isolating and initiating primary culture of PGCs from 6-7-day-old tva r2/r2 and tvb r3/r3 genotype QYPC embryos. (a) Fertile eggs and QYPCs with tva r2/r2 and tvb r3/r3 genotypes; (b) Embryos of 6-7 days old tva r2/r2 and tvb r3/r3 genotypes QYPCs; (c) a pair of male gonads attached to the surface of the kidney; (d) Several gonadal tissues that have been stripped clean; (e) somatic cells and PGCs after 5 h of differential adhesion; (f) Transfer the post-pasted PGCs to the cell culture plate for primary culture; (B) The Fig. shows the general process of co-culturing PGCs <t>with</t> <t>STO</t> cells for line establishment. (a) The cell morphology of PGCs-STO cells in primary co-culture for 4 days; (b) The cell morphology of PGCs-STO cells cocultured for 8 days; (c) The cell morphology of PGCs-STO cells cocultured for 14 days; (d) The cell morphology of PGCs-STO cells cocultured for 21days and successfully constructed.
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Effects of RSV on mitochondrial function–related indicators exposed to TCS. (A, B) Protein expression and quantitative analysis of SIRT1, PGC–1α, NRF1, and mRNA expression levels (C) mRNA expression levels of SIRT1, PGC–1α, NRF1, and TFAM (D) mRNA expression levels of MFN2, OPA1, MFN1, DRP1, FIS1, and MFF. Data were analyzed using one–way ANOVA. Inter–group differences are indicated by different letters ( P < 0.05). Groups with the same letter were considered not to have significant differences (mean ± SD, n = 6).

Journal: Poultry Science

Article Title: Resveratrol protects against triclosan–induced impairment of colonic barrier function in chickens

doi: 10.1016/j.psj.2026.106946

Figure Lengend Snippet: Effects of RSV on mitochondrial function–related indicators exposed to TCS. (A, B) Protein expression and quantitative analysis of SIRT1, PGC–1α, NRF1, and mRNA expression levels (C) mRNA expression levels of SIRT1, PGC–1α, NRF1, and TFAM (D) mRNA expression levels of MFN2, OPA1, MFN1, DRP1, FIS1, and MFF. Data were analyzed using one–way ANOVA. Inter–group differences are indicated by different letters ( P < 0.05). Groups with the same letter were considered not to have significant differences (mean ± SD, n = 6).

Article Snippet: PGC-1α , 1:600 , WANLEIBIO.

Techniques: Expressing

In vitro isolation and culture process of PGCs from QYPCs with tva r2/r2 and tvb r3/r3 genotypes. (A) The process of isolating and initiating primary culture of PGCs from 6-7-day-old tva r2/r2 and tvb r3/r3 genotype QYPC embryos. (a) Fertile eggs and QYPCs with tva r2/r2 and tvb r3/r3 genotypes; (b) Embryos of 6-7 days old tva r2/r2 and tvb r3/r3 genotypes QYPCs; (c) a pair of male gonads attached to the surface of the kidney; (d) Several gonadal tissues that have been stripped clean; (e) somatic cells and PGCs after 5 h of differential adhesion; (f) Transfer the post-pasted PGCs to the cell culture plate for primary culture; (B) The Fig. shows the general process of co-culturing PGCs with STO cells for line establishment. (a) The cell morphology of PGCs-STO cells in primary co-culture for 4 days; (b) The cell morphology of PGCs-STO cells cocultured for 8 days; (c) The cell morphology of PGCs-STO cells cocultured for 14 days; (d) The cell morphology of PGCs-STO cells cocultured for 21days and successfully constructed.

Journal: Poultry Science

Article Title: Generation of chimeric chickens resistant to infection by avian leukemia virus subgroups A, B, D, E, and K through primordial germ cells

doi: 10.1016/j.psj.2026.106642

Figure Lengend Snippet: In vitro isolation and culture process of PGCs from QYPCs with tva r2/r2 and tvb r3/r3 genotypes. (A) The process of isolating and initiating primary culture of PGCs from 6-7-day-old tva r2/r2 and tvb r3/r3 genotype QYPC embryos. (a) Fertile eggs and QYPCs with tva r2/r2 and tvb r3/r3 genotypes; (b) Embryos of 6-7 days old tva r2/r2 and tvb r3/r3 genotypes QYPCs; (c) a pair of male gonads attached to the surface of the kidney; (d) Several gonadal tissues that have been stripped clean; (e) somatic cells and PGCs after 5 h of differential adhesion; (f) Transfer the post-pasted PGCs to the cell culture plate for primary culture; (B) The Fig. shows the general process of co-culturing PGCs with STO cells for line establishment. (a) The cell morphology of PGCs-STO cells in primary co-culture for 4 days; (b) The cell morphology of PGCs-STO cells cocultured for 8 days; (c) The cell morphology of PGCs-STO cells cocultured for 14 days; (d) The cell morphology of PGCs-STO cells cocultured for 21days and successfully constructed.

Article Snippet: PGCs on STO feeder layers in 24-well plates were fixed with 4% paraformaldehyde (Servicebio, G1101) for 30 min, then washed three times with PBS (Servicebio, G4202).

Techniques: In Vitro, Isolation, Cell Culture, Co-Culture Assay, Construct